Human PRKN Protein, Recombinant

产品货号：PR00262HuP1
   
$ 询价

规格    100ug

产品名称：Human PRKN Protein, Recombinant
 
纯度：≥95 % as determined by SDS-PAGE
 
内毒素：/
 
生物活性：/
 
序列起止：Ile229~Val465
 
标签：N-His tag
 
Uniprot链接：O60260
 
表达系统：E.coli
 
种属：Human
 
预测 N 端：Met
 
预测分子量：/
 
缓冲液：20mM Tris, 250mM imidazole, 500mM NaCl, pH8.0, containing 10% glycerol.
 
运输方式：This Protein is shipped as lyophilized powder at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
稳定性 & 储存条件：Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
 
复溶：Please refer to COA for detailed information
 
质量验证图：/

别称：AR-JP, LPRS2, PDJ, PRKN, Parkin, Autosomal Recessive,Juvenile, E3 ubiquitin-protein ligase parkin
 
背景信息：Parkin. The E3 Ubiquitin ligase Parkin (encoded by the PARK2 gene) is an essential part of the cellular machinery that participates in the removal of damaged mitochondria. Mutations in PARK2 are known to cause a form of Parkinson's disease known as autosomal recessive juvenile Parkinson's disease (AR-JP), and the mechanisms by which defective Parkin ligase contributes to the dopaminergic cell death in this disease is an area of intense investigation.Reported substrates for Parkin include BCL2, GPR37, MIRO1, MFN1, MFN2, TOMM20, USP30, and many others. Parkin (an RBR-class Ubiquitin ligase) structures have recently been reported by multiple groups, and reveal that the ligase is folded upon itself to produce an auto-inhibited state. The auto-inhibition is relieved by interactions with PINK1 kinase (which can phosphorylate both Parkin and Ubiquitin at serine residue number 65) and pS65 phospho-Ubiquitin by mechanisms that are under investigation.In vitro, wild-type Parkin may be activated by treatment with recombinant PINK1, or addition of low concentrations of pS65-phosphoubiquitin.  Parkin has been reported to generate poly-Ubiquitin chains in K6, K11, K48, and K63 linkages both in vitro and in vivo.  This recombinant protein is untagged, and is not active as determined by an autoubiquitination assay--it is intended as a negative control.

全称：E3 ubiquitin-protein ligase parkin (PRKN)

说明书：待上传