Human LGMN Protein, Recombinant

产品货号：PR00396HuP1
   
$ 询价

规格    100ug

产品名称：Human LGMN Protein, Recombinant
 
纯度：≥95 % as determined by SDS-PAGE
 
内毒素：/
 
生物活性：/
 
序列起止：Val18~Asn323
 
标签：C-His tag
 
Uniprot链接：Q99538
 
表达系统：E.coli
 
种属：Human
 
预测 N 端：Met
 
预测分子量：33.66 kDa
 
缓冲液：20mM Tris, 250mM imidazole, 500mM NaCl, pH8.0, containing 10% glycerol.
 
运输方式：This Protein is shipped as lyophilized powder at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
稳定性 & 储存条件：Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
 
复溶：Please refer to COA for detailed information
 
质量验证图：/

别称：AEP, LGMN1, PRSC1, Protease,Cysteine 1, Asparaginyl endopeptidase
 
背景信息：Legumain/Asparaginyl Endopeptidase. Legumain is a lysosomal cysteine protease whose activity is found in several tissues tested (1, 2). Legumain plays a pivotal role in the endosomal/lysosomal degradation system because the Legumain deficiency causes the accumulation of pro cathepsins B, H and L, another group of lysosomal cysteine proteases (3). Over-expression of Legumain in tumors is significant for invasion/metastasis (4). Also known as Asparaginyl Endopeptidase, it specifically cleaves peptide bonds with Asn at the P1 position. Nevertheless, it also cleaves peptide bonds with Asp at the P1 position. Auto-activation of pro Legumain involves both types of the cleavage, which result in the removal of the pro peptides in both C- and N-termini (5). In addition, Legumain activates pro MMP-2 and processes bacterial antigens for MHC class II presentation and pro thymosin  alpha  to thymosin  alpha 1 and thymosin  alpha 11, two acidic peptides with immunoregulatory properties (6‑8). Human Legumain is synthesized as a 433 amino acid precursor with a signal peptide (residues 1‑17). The pro enzyme (residues 18‑433) was expressed with an N-terminal His tag. This activity of Legumain can be inhibited by recombinant human Cystatins C and E/M and recombinant mouse Cystatin C (Catalog # 1196-PI, 1286-PI and 1238-PI, respectively).

全称：Legumain (LGMN)

说明书：待上传