Human Angiotensin-converting enzyme 2 (ACE2) Protein, Recombinant
产品货号：PR00303HuP1
$ 询价
规格 100ug
浓度 230ug/ml
产品名称：Human Angiotensin-converting enzyme 2 (ACE2) Protein, Recombinant
状态：Lyophilized
纯度：>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
内毒素：<0.01 EU per 1 μg of the protein by the LAL method
生物活性：The specific activity is 500 pmol/min/µg
序列起止：Lys475~Lys773
Uniprot链接：Q9BYF1
宿主：Bacteria
种属：Human
预测分子量：31 kDa
实际分子量：31kDa as determined by SDS-PAGE reducing conditions.
稳定性 & 储存条件：Store for up to one year at -20°C to -80°C as lyophilized powder.Please avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:-20°C to -80°C for 12 months in lyophilized state;-80°C for 3 months under sterile conditions after reconstitution.
复溶：Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA. Please contact us for any concerns or special requirements.Note:When reconstituting,gently pipet and wash down the sides of the vial .Do not vortex.
质量验证图：
Figure1. SDS-PAGE
For research use only. Not for drug, in vitro, household or other uses.
别称：ACEH, ACEII, ACE-II, Peptidyl-Dipeptidase A, ACE-related carboxypeptidase, Angiotensin-converting enzyme homolog, Metalloprotease MPROT15
背景信息：ACE-2. Angiotensin I Converting Enzyme (ACE-2), also called ACEH (ACE homologue), is a dimeric, zinc-dependent metalloprotease of the ACE family that also includes somatic and germinal ACE (1, 2). ACE-2 mRNA is found at high levels in heart, testis, and kidney and at lower levels in a wide variety of tissues (1, 3). ACE-2 is the SARS-CoV and SARS-CoV2 Spike protein receptor in vivo (4-6), functions catalytically as a carboxypeptidase to cleave several substrates including angiotensins I and II, and acts as a partner for B0AT1-family amino acid transporters (1, 2). Through these functions, ACE-2 has been shown to be involved in several diseases including SARS, COVID19, acute lung injury (4, 7), heart disease (8), liver and lung fibrosis (9), inflammatory lung disease (10), and cardiopulmonary disease (11). Full length ACE-2 protein includes an extracellular region composed of a single N-terminal peptidase domain and C-terminal collectrin-like domain (CLD), a transmembrane domain, and a short cytoplasmic tail (12). The N-terminal peptidase region is required for binding to SARS-CoV and SARSCoV2 spike proteins, while the CLD contains a region that promotes dimerization and association with amino acid transporters (2). The peptidase domain contains a long deep cleft that undergoes a large hinge-bending movement at substrate and inhibitor binding (12). Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity and inhibitors of ACE-2 do not inhibit ACE activity (13).
全称：Angiotensin-converting enzyme 2 (ACE2)
说明书：待上传