Human GAPDH Protein, Recombinant
产品货号:PR00002HuP1
$ 询价
规格 100μg
产品名称:Human GAPDH Protein, Recombinant
纯度:≥95 % as determined by SDS-PAGE
内毒素:/
生物活性:/
序列起止:Gly2~Glu335
标签:N-His tag
Uniprot链接:P04406
表达系统:E.coli
种属:Human
预测 N 端:Met
预测分子量:36.74 kDa
缓冲液:20mM Tris, 250mM imidazole, 500mM NaCl, pH8.0, containing 10% glycerol.
运输方式:This Protein is shipped as lyophilized powder at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
稳定性 & 储存条件:Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
复溶:Please refer to COA for detailed information
别称:G3PD, GAPD, Peptidyl-cysteine S-nitrosylase GAPDH
背景信息:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
全称:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)
说明书:待上传