Human CASP2 Protein, Recombinant

产品货号：PR00546HuP1
   
$ 询价

规格：100ug

产品名称：Human CASP2 Protein, Recombinant
 
纯度：≥95 % as determined by SDS-PAGE
 
内毒素：/
 
生物活性：/
 
序列起止：Gly170~Asp333
 
标签：C-His tag
 
Uniprot链接：P42575
 
表达系统：E.coli
 
种属：Human
 
预测 N 端：Met
 
预测分子量：/
 
缓冲液：20mM Tris, 250mM imidazole, 500mM NaCl, pH8.0, containing 10% glycerol.
 
运输方式：This Protein is shipped as lyophilized powder at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
稳定性 & 储存条件：Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
 
复溶：Please refer to COA for detailed information
 
别称：ICH1L, NEDD2, ICH-1L/1S, Cysteinyl Aspartate Specific Proteinases 2, Apoptosis-Related Cysteine Peptidase, Neural Precursor Cell Expressed Developmentally Down-Regulated 2
 
背景信息：Caspase-2. Caspase-2 (Cysteine-aspartic acid protease 2/Casp2; also NEDD2 and ICH-1) is a 30‑32 kDa member of the peptidase C14A/IL‑1 beta ‑converting family of enzymes (1‑3). It is widely expressed and is an integral component of the apoptotic cascade. Based on the length of its prodomain, caspase-2 has been considered to be an initiator caspase. However, studies have shown that other caspases (such as Casp 3) activate procaspase 2, and Caspase-2 likely acts on key cellular molecules such as BID, Golgin 160 and DFF45/ICAD (2, 4, 5). Thus, Caspase-2 is perhaps more likely to be a specialized executioner caspase. Human procaspase-2 is a 48‑51 kDa, 452 amino acid (aa) protein (4‑7). It is known to exist as a disulfide-linked homodimer via covalent linkage at Cys436 (2, 5). But this dimeric state may not be sufficient for (auto)activation. Actual activation may occur following oligomerization within the context of activating platforms such as DISC (death-inducing signaling complex) or the PIDDosome (8‑10). Initially, procaspase-2 undergoes proteolytic cleavage to generate an N-terminal 333 aa p34/34 kDa subunit, and a 119 aa C-terminal p14/14 kDa subunit (5). The p34 and p14 subunits are further processed to generate the prodomain (aa 1‑169), plus the mature p18 (aa 170‑333) and p12 (aa 348‑452) subunits (4‑6). Notably, each p18:p12 noncovalent heterodimer demonstrates proteolytic activity around a catalytic site at aa 318‑322, and, due to an nuclear localization signal within the prodomain, may be found in either nucleus or cytoplasm (11, 12). There are multiple potential isoform variants. Individually, or in combination, there is an alternative start site at Met18, a substitution of two aa for aa 107‑452, a second substitution of 14 aa for aa 309‑322, and a third substitution of 22 aa for aa 323‑452 (6, 7, 13). The human and mouse procaspase 2 precursors are 90% aa identical, with the majority of differences lying in the prodomain.
 
全称：Caspase-2 (CASP2)
 
说明书：待上传