Human GALNT4 Protein, Recombinant

产品货号:PR00561HuP1
   
询价

规格:100ug

产品名称Human GALNT4 Protein, Recombinant
 
纯度≥95 % as determined by SDS-PAGE
 
内毒素/
 
生物活性/
 
序列起止Met36~Lys578
 
标签/
 
Uniprot链接Q8N4A0
 
表达系统E.coli
 
种属Human
 
预测 N 端Met
 
预测分子量/
 
缓冲液20mM Tris, 250mM imidazole, 500mM NaCl, pH8.0, containing 10% glycerol.
 
运输方式This Protein is shipped as lyophilized powder at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
稳定性 & 储存条件Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
 
复溶Please refer to COA for detailed information
 
别称pp-GaNTase 4
 
背景信息Polypeptide GalNAc Transferase 4/GALNT4. O-glycosylation is a ubiquitous post-translational modification present in secreted and membrane-bound proteins. Polypeptide N-acetylgalactosaminyltransferases (GALNTs) calalyze the initial step for O-glycosylation by transferring GalNAc to Thr or Ser residues (GalNAc  alpha 1-O-Ser/Thr) in the Golgi compartment. Structurally, the GALNTs consist of an N-terminal catalytic domain tethered by a short linker to a C-terminal ricin-like lectin domain containing three potential carbohydrate-binding sites (1, 2). Twenty distinct GALNT isoforms have been detected in humans. These isoforms display both unique and overlapping substrate specificities (3, 4, 5) with no known universal consensus glycosylation sequence. Glycosylation of mucins results from the successive, often hierarchical, action of several specific GALNTs (6). Human GALNT4 is widely expressed with particularly high expression in the immune system (7). The enzymatic specificity of GALNT4 depends on the selective binding between the C-terminal lectin domain and the existing O-GalNAc modified residues present within the substrate polypeptide (8). Along with GALNT3, GALNT4 is classified as an intermediate transferase that glycosylates mucin polypeptides that have been previously mono- or di-glycosylated by other transferases (6). The enzymatic activity of recombinant human GALNT4 was determined using a phosphatase-coupled assay (9).
 
全称Polypeptide N-acetylgalactosaminyltransferase 4 (GALNT4)
 
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