Human VEGF165 Protein, Recombinant

产品货号：PR00787HuP1
   
$ 询价

规格：100ug

产品名称：Human VEGF165 Protein, Recombinant
 
纯度：≥95 % as determined by SDS-PAGE
 
内毒素：/
 
生物活性：/
 
序列起止：Ala27~Arg191
 
标签：C-His tag
 
Uniprot链接：P15692
 
表达系统：E.coli
 
种属：Human
 
预测 N 端：Met
 
预测分子量：/
 
缓冲液：20mM Tris, 250mM imidazole, 500mM NaCl, pH8.0, containing 10% glycerol.
 
运输方式：This Protein is shipped as lyophilized powder at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
稳定性 & 储存条件：Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
 
复溶：Please refer to COA for detailed information
 
别称：/
 
背景信息：VEGF. The human VEGF-A gene contains eight exons plus a nontraditional ninth exon in the 3’ UTR (1). The A gene gives rise to multiple splice variants of VEGF-A, ranging from 121 amino acids (aa) to 206 aa in length. All human splice variants contain exons 1-5 and some part of exon 8. Exons 6 and 7 are split into at least two parts, some of which may be included in any one molecule (1-5). The nontraditional 9th exon is a substitute for exon 8. Incorporation of the 9th exon results in a non-signaling form of VEGF (5). VEGF162 is a VEGF isoform that induces endothelial cell proliferation in vitro and angiogenesis in vivo (5). It is synthesized as a 40-45 kDa disulfide-linked homodimer whose subunits are a product of exons 1-5, 6a, 6b and 8 (5). The three aa difference between VEGF165 and VEGF162 is attributable to the fact that the 165 form only utilizes exon 7 while the 162 form only utilizes exon 6. Within exon 6, exon 6a contributes 24 aa while exon 6b contributes 17 aa (aa 116‑156 in the mature segment) (2). Exon 6a is not commonly found with exon 6b. In VEGF162, exon 6b appears to act as a counterpart to the activity of exon 6a. That is, the product of exon 6a normally promotes VEGF binding to basement membrane while the product of exon 6b promotes VEGF dissociation and solubilization. In this case, VEGF162 is notably soluble.
 
全称：Vascular Endothelial Growth Factor 165 (VEGF165)
 
说明书：待上传