Human NGF Protein, Recombinant

产品货号：PR00810HuP1
   
$ 询价

规格：100ug

产品名称：Human NGF Protein, Recombinant
 
纯度：≥95 % as determined by SDS-PAGE
 
内毒素：/
 
生物活性：/
 
序列起止：Ser122~Ala241
 
标签：C-His tag
 
Uniprot链接：P01138
 
表达系统：E.coli
 
种属：Human
 
预测 N 端：Met
 
预测分子量：/
 
缓冲液：20mM Tris, 250mM imidazole, 500mM NaCl, pH8.0, containing 10% glycerol.
 
运输方式：This Protein is shipped as lyophilized powder at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
稳定性 & 储存条件：Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
 
复溶：Please refer to COA for detailed information
 
别称：NGFB, Beta-NGF, HSAN5, NGF-B, Beta-Nerve Growth Factor
 
背景信息：beta-NGF. NGF was discovered as a molecule that promoted the survival and differentiation of sympathetic and sensory neurons in the peripheral nervous system (1). In addition,  beta -NGF can act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons (2).  beta -NGF has also been shown to have biological effects on a variety of neurons, glia, and nonneural cells (3). NGF was initially isolated from the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits,  alpha,  beta, and  gamma. It is now known that both the  alpha  and  gamma  subunits of NGF are members of the kallikrein family of serine proteases while the  beta  subunit, called  beta -NGF, exhibits all the biological activities ascribed to NGF (4 - 5). Recombinant mouse  beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human  beta -NGF and 95.8% with rat  beta -NGF (6).NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). NT-6 and NT-7 were also identified in fish (7, 8). Biological activities of the NGF family are mediated through binding and activation of two types of receptors, TrKs (TrKA, TrKB, and TrKC) and NGF receptor. TrKs are single-pass membrane proteins belonging to the receptor tyrosine kinase family. The neurotrophins bind to TrKs specifically. NGF is specific for TrKA, BDNF and NT-4 for TrKB, and NT-3 mostly for TrKC. NGF receptor is a TNF receptor superfamily protein that binds to all the neurotrophins with similar affinity (9). TrkA is often referred to as the high-affinity receptor. However, most high affinity binding is mediated by the interaction of NGF receptor with TrKA. Both NGF and its receptors are expressed during development, adult life and aging in many cell types in CNS and PNS, immune and inflammatory systems. NGF signaling has been shown to play an important role in neuroprotection and repair (3, 10).
 
全称：Beta-nerve growth factor (NGF)
 
说明书：待上传