Human IGF2 Protein, Recombinant

     
产品货号：PR00015HuP1
   
$ 询价

规格     100μg

产品名称：Human IGF2 Protein, Recombinant
 
纯度：≥95 % as determined by SDS-PAGE
 
内毒素：/
 
生物活性：/
 
序列起止：Ala25~Glu91
 
标签：N-His tag
 
Uniprot链接：P01344
 
表达系统：E.coli
 
种属：Human
 
预测 N 端：Met
 
预测分子量：7.37 kDa
 
缓冲液：20mM Tris, 250mM imidazole, 500mM NaCl, pH8.0, containing 10% glycerol.
 
运输方式：This Protein is shipped as lyophilized powder at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
稳定性 & 储存条件：Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
 
复溶：Please refer to COA for detailed information
 
别称：INSIGF, IGF-II, Somatomedin A, Preptin, Insulin-like growth factor II Ala-25 Del
 
背景信息：IGF-II/IGF2. IGF-II (Insulin-like growth factor II; also multiplication-stimulating polypeptide/MSP and somatomedin-A) is a secreted 8 kDa polypeptide that belongs to the insulin family of peptide growth factors (1, 2, 3). It is part of a complex system of growth and metabolic-regulating proteins that is particularly important during development. It has been associated with nervous system proliferation and differentiation, myelination, adrenal cortical proliferation, and skeletal growth and differentiation (4). In human, IGF-II is primarily synthesized by the liver, and circulates at high levels in both fetus and adult. In rodent, however, IGF-II levels drop after the perinatal period, an effect attributed to the lack of a key gene promoter (2, 5). This may indicate that postnatally, IGF-II has either a limited, or local effect only in rodent. For example, evidence suggests IGF-II may be the intermediary for SHH induction of VEGF attendant with local neovascularization (6). Rodent cells known to express IGF-II include astrocytes (7), hepatocytes (8), osteoblasts (9), embryonic striated muscle cells (10, 11) plus Kupffer cells and Ito cells (12). Mouse IGF-II is synthesized as a 180 amino acid (aa) preproprecursor (13). It contains a 24 aa signal sequence, a 67 aa mature region, and an 89 aa C-terminal prodomain that is alternatively referred to as the E-peptide. Mature IGF-II is 91% and 97% aa identical to human and rat IGF-II, respectively. Proper processing of IGF-II requires the chaperone activity of GRP94 (14). This generates an 8 kDa mature form, an 18 kDa, 156 aa proform, and a potential 11 kDa, 88 aa “Big” form (aa 25-112).  This 11 kDa ”Big” form would be equivalent to human 15-16 kDa IGF-II, with the 5 kDa difference attributable to the presence of O-linked glycosylation (15).  There is an additional 34 aa proteolytic fragment that is termed preptin and contains aa 93-126 of the preproprecursor.  This is distinct from IGF-II, is secreted by pancreatic b cells, and facilitates insulin secretion (16, 17). IGF-II has multiple binding partners.  It binds to IGF-IR, the Insulin receptor (IR)-type A and IGF-IR:IR-A hybrids, the type 2 IGF receptor (IGF-2R), and IGF binding proteins 1-6 (18, 19).  The first three receptors initiate downstream signaling events, the IGF-2R sequesters local IGF-II, and the six IGFBPs regulate IGF-II activity in various tissues.
 
全称：Insulin-like growth factor II (IGF2)
 
说明书：待上传