Human IL2 Protein, Recombinant

产品货号：PR00020HuP1
   
$ 询价

规格    100ug

产品名称：Human IL2 Protein, Recombinant
 
纯度：≥95 % as determined by SDS-PAGE
 
内毒素：/
 
生物活性：/
 
序列起止：Ala21~Thr153
 
标签：N-His tag
 
Uniprot链接：P60568
 
表达系统：E.coli
 
种属：Human
 
预测 N 端：Met
 
预测分子量：14.63 kDa
 
缓冲液：20mM Tris, 250mM imidazole, 500mM NaCl, pH8.0, containing 10% glycerol.
 
运输方式：This Protein is shipped as lyophilized powder at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
稳定性 & 储存条件：Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
 
复溶：Please refer to COA for detailed information
 
质量验证图：/

别称：TCGF, Lymphokine, T-Cell Growth Factor, Aldesleukin
 
背景信息：IL-2. Bovine Interleukin-2 (IL-2) is a 15 kDa,  alpha -helical, single chain, potentially glycosylated polypeptide that has potent stimulatory activity for antigen-activated T cells (1 - 5). The molecule is synthesized as a 155 amino acid (aa) precursor that contains a 20 aa signal peptide plus a 135 aa mature segment that is possibly O-glycosylated (4, 5). The mature region has multiple  alpha -helices and one intrachain disulfide bond. Mature bovine IL-2 is 64%, 60%, 49%, 50%, 72%, 63% and 67% to mature human, canine, mouse, rat, porcine, equine, and feline IL-2, respectively. Mammalian cells known to express IL-2 include CD4+ and CD8+ T cells, visceral smooth muscle cells, eosinophils,  gamma δ T cells, B cells and dendritic cells. The receptor for IL-2 is complex and consists of three distinct subunits in varying combinations (6, 7). Two of these are ligand-binding and are termed IL-2 R alpha  and IL-2 R beta. IL-2 R alpha  is 55 kDa and binds IL-2 with low affinity. IL-2 R beta  is 75 kDa and binds IL-2 with intermediate affinity. Signal transduction is performed by both IL-2 R beta  and a 64 kDa common gamma chain ( gamma c). This signal transducing common gamma chain does not bind IL-2, but does heterodimerize with IL-2 R beta  to form a functional IL-2 receptor. The complex heterotrimeric  alpha -beta -gamma c receptor may arise from IL-2 binding to preformed R alpha -R beta  complexes (8). Functionally, IL-2 is best known for its autocrine and paracrine activity on T cells. It drives resting T cells into active G1, inducing IL-2 and IL-2 R alpha  synthesis and cell proliferation (7). It also promotes Fas-induced death of naïve CD4+ T cells, while having minimal effect on activated CD4+ memory lymphocytes. Finally, IL-2 seems to play a central role in the expansion and maintenance of CD4+ CD25+ regulatory T cells. Thus, IL-2 may be a key cytokine in the natural suppression of autoimmunity (9, 10).

全称：Interleukin-2 (IL2)
 
说明书：待上传