产品名称：Human FCER2 Protein, Recombinant
纯度：≥95 % as determined by SDS-PAGE
内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
生物活性：/
序列起止：Met150~Ser321
标签：N-His tag
Uniprot链接：P06734
表达系统：293F
种属：Human
预测 N 端：Met
预测分子量：/
缓冲液：20mM Tris, 250mM imidazole, 500mM NaCl, pH8.0, containing 10% glycerol.
运输方式：This Protein is shipped as lyophilized powder at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
稳定性 & 储存条件：Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
复溶：Please refer to COA for detailed information
质量验证图：/
别称：FceRII, FCER2, BLAST-2, CLEC4J, FCE2, IGEBF, Receptor II For The Fc Region Of Immunoglobulin E, C-type lectin domain family 4 member J, Immunoglobulin E-binding factor, Lymphocyte IgE receptor
背景信息：CD23/Fc epsilon RII. CD23 (also named B cell differentiation antigen) is a member of subgroup II of the C-type (Ca++-dependent) lectin superfamily (1 - 5). Human CD23 is a 47 kDa, type II transmembrane glycoprotein that is expressed by a wide variety of cell types (6 - 10). The full-length receptor is 321 amino acids (aa) in length and contains a 274 aa extracellular region, a 26 aa transmembrane segment, and a 21 aa cytoplasmic domain. The extracellular region contains a C-type lectin domain and a connecting stalk with coiled-coil topography (3, 11). The lectin domain binds both protein and carbohydrate in an apparently Ca++ independent manner (11). The coiled-coil region contributes to oligomerization (11, 12). The lectin domain in human CD23 (aa 162 - 284) is 64%, 62% and 68% aa identical to the lectin domains in mouse, rat and bovine CD23, respectively. In the cytoplasmic region, two FC isoforms exist which arise from alternate start sites (6, 12). The “a” (or long) isoform begins with the sequence MEEGQYS and is constitutively expressed by B cells. It is believed to participate in IgE-mediated endocytosis (13). The “b” (or short) isoform begins with MNPPSQ and is induced on a wide variety of cell types by IL-4 (6). Fcb reportedly contributes to IgE-mediated phagocytosis (13). Fcb expressing cells include eosinophils, monocytes, visceral smooth muscle and intestinal epithelium (6, 14, 15). At least four soluble forms of CD23 are known to exist. They range in molecular weight from 25 kDa to 37 kDa, with the 25 kDa form predominating in sera (16). Soluble CD23 (sFc) is generated by metalloprotease (ADAM8; ADAM15; ADAM28) and cysteine-protease activity (16 - 18). Cleavage usually occurs between aa 150 - 160 (7, 8). It is unclear if sequential metalloprotease-cysteine protease activity is necessary for the generation of all soluble forms. Both soluble and membrane-bound CD23 show bioactivity. Ligands for CD23 include CD21, IgE, CD11b, and CD11c (19 - 21). CD23 binding to CD11b and Cd11c on monocytes results in oxidative product generation and proinflammatory cytokine release (21). On B cells, sCD23 induces IgE secretion by binding CD21. Conversely, secreted IgE will, in turn, bind B cell membrane CD23, rendering it unavailable for cleavage, and thus shutting down IgE production (11).
全称：Low affinity immunoglobulin epsilon Fc receptor (FCER2)