Human GALNT4 Protein, Recombinant

产品货号：PR00561HuM1
   
$ 询价

规格    100ug

产品名称：Human GALNT4 Protein, Recombinant
 
纯度：≥95 % as determined by SDS-PAGE
 
内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
 
生物活性：/
 
序列起止：Ala36~Lys578
 
标签：N-His tag
 
Uniprot链接：Q8N4A0
 
表达系统：CHOS
 
种属：Human
 
预测 N 端：Met
 
预测分子量：/
 
缓冲液：20mM Tris, 250mM imidazole, 500mM NaCl, pH8.0, containing 10% glycerol.
 
运输方式：This Protein is shipped as lyophilized powder at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
稳定性 & 储存条件：Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
 
复溶：Please refer to COA for detailed information
 
质量验证图：/

别称：pp-GaNTase 4
 
背景信息：Polypeptide GalNAc Transferase 4/GALNT4. O-glycosylation is a ubiquitous post-translational modification present in secreted and membrane-bound proteins. Polypeptide N-acetylgalactosaminyltransferases (GALNTs) calalyze the initial step for O-glycosylation by transferring GalNAc to Thr or Ser residues (GalNAc  alpha 1-O-Ser/Thr) in the Golgi compartment. Structurally, the GALNTs consist of an N-terminal catalytic domain tethered by a short linker to a C-terminal ricin-like lectin domain containing three potential carbohydrate-binding sites (1, 2). Twenty distinct GALNT isoforms have been detected in humans. These isoforms display both unique and overlapping substrate specificities (3, 4, 5) with no known universal consensus glycosylation sequence. Glycosylation of mucins results from the successive, often hierarchical, action of several specific GALNTs (6). Human GALNT4 is widely expressed with particularly high expression in the immune system (7). The enzymatic specificity of GALNT4 depends on the selective binding between the C-terminal lectin domain and the existing O-GalNAc modified residues present within the substrate polypeptide (8). Along with GALNT3, GALNT4 is classified as an intermediate transferase that glycosylates mucin polypeptides that have been previously mono- or di-glycosylated by other transferases (6). The enzymatic activity of recombinant human GALNT4 was determined using a phosphatase-coupled assay (9).

全称：Polypeptide N-acetylgalactosaminyltransferase 4 (GALNT4)
 
说明书：待上传