产品名称:Anti-FLAG Antibody, Mouse Monoclonal, F1F
克隆号:F1F
抗体亚型:/
经验证的应用:WB
交叉反应:Predict reacts with: Human/Mouse/Rat
特异性:Flag-tag
免疫原:Synthetic Peptide
制备方法:Produced in mouse immunized with FLAG, and purified by antigen affinity chromatography.
来源:Monoclonal Mouse IgG
纯化:Immunogen affinity purified
缓冲液:Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物:Unconjugated
浓度:Liquid
运输方式:This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片:
Figure1.Application in WB
Western blot analysis of extracts of various lane1 10ng recombinant proteins containing the Flag Tag、lane2 50ng recombinant proteins containing the Flag Tag, using FLAG antibody (TM00975GeM20-F1F) at 1μg/mL. Secondary antibody: Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:4000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1.5min.
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