产品名称：Anti-ACTB Antibody, Mouse Monoclonal, B7T
克隆号：B7T
抗体亚型：/
经验证的应用：WB/IHC
交叉反应：Human/Mouse/Rat
特异性：endogenous levels of human ACTB.
免疫原：A synthetic peptide of human ACTB
制备方法：Produced in mouse immunized with ACTB, and purified by antigen affinity chromatography.
来源：Monoclonal Mouse IgG
纯化：Immunogen affinity purified
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物：Unconjugated
浓度：Liquid
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片：
Figure1.Application in WB
Western blot analysis of extracts of various cell lines and tissues, using ACTB antibody (CM00001HuM10-B7T) at 1μg/mL Secondary antibody: HRP Mouse Anti-Mouse IgG (H+L) at 1：20000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0.1s.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Monkey left ventricle、Monkey liver、Monkey prostate、Monkey spleen、Monkey jejunum、Monkey epididymis sections labelling ACTB with purified CM00001HuM10-B7T at 5ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1：2000 dilution. PBS instead of the primary antibody was used as the negative control.
别称：Beta-actin
背景信息：Beta Actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40, 31, 15 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. (9173887, 11217076, 10229193 )
全称：Actin, cytoplasmic 1 (ACTB)