Anti-ACTA1 Antibody, Mouse Monoclonal, E9T

产品编号：CM01105HuM10-E9T
   
$ 询价

规格     100ul
 

产品名称：Anti-ACTA1 Antibody, Mouse Monoclonal, E9T
 
克隆号：E9T
 
抗体亚型：/
 
经验证的应用：/
 
交叉反应：Predict reacts with: Human/Mouse/Rat
 
特异性：/

免疫原：A synthetic peptide of human ACTA1
 
制备方法：Produced in mouse immunized with ACTA1, and purified by antigen affinity chromatography.
 
来源：Monoclonal Mouse IgG
 
纯化：Immunogen affinity purified
 
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物：Unconjugated
 
浓度：Liquid
 
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片：






Figure1.Western blot analysis of extracts of various lane1 Rat brain(+++)、lane2 Mouse brain(+++), using ACTA1 antibody (CM01105HuM10-E9T) at 1ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1：20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 8min.











 


Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Monkey prostate、Monkey submandibular gland、Monkey skeletal muscle、Monkey kidney、Monkey lung、Monkey epididymis sections labelling ACTA1 with purified CM01105HuM10-E9T at 5ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1：2000 dilution. PBS instead of the primary antibody was used as the negative control.

 

别称：ACTA, Alpha-actin-1, Actin, alpha skeletal muscle, intermediate form
 
背景信息：Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms.

全称：Actin, alpha skeletal muscle (ACTA1)
 
说明书：待上传