产品名称：Anti-H3C1 Antibody, Rabbit Polyclonal
经验证的应用：WB/ICC
交叉反应：/
特异性：human H3C1
免疫原：Recombinant human H3C1 protein, fragment Met1~Ala136; UniprotKB: P68431
制备方法：Produced in rabbits immunized with human H3C1, and purified by antigen affinity chromatography.
来源：Polyclonal Rabbit IgG
纯化：Immunogen affinity purified
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物：Unconjugated
状态：Liquid
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片：
Figure1.Western blot analysis of extracts of various cell lines using H3C1 antibody (CP00037HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 4s.
Figure2.Western blot analysis of extracts of various cell lines using H3C1 antibody (CP00037HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10s.
Figure3.Western blot analysis of extracts of various cell lines, using H3C1 antibody (CP00037HuA10) at 5 ug/ml.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 21s.
Figure4.Immunocytochemistry analysis of Hela(Human cervical cancer cell) cells labeling H3C1 with purified CP00037HuA10 at 5 µg/mL. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with CY3 conjugated Anti-mouse IgG(H+L) (GB21301) 1/200 (2.5 µg/mL). Goat anti- mouse IgG (Alexa Fluor® 488, GB25301) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
别称：Histone H3/a
背景信息：/
全称：Histone H3.1 (H3C1)