Anti-ACTA2 Antibody, Mouse Monoclonal, A1T

产品编号:CM00526HuM10-A1T
   
询价

规格     100ul

说明书
 

产品名称Anti-ACTA2 Antibody, Mouse Monoclonal, A1T
 
克隆号A1T
 
抗体亚型:/
 
经验证的应用WB/IHC
 
交叉反应Predict reacts with: Human/Mouse/Rat
 
特异性:/

免疫原A synthetic peptide of human ACTA2
 
制备方法Produced in mouse immunized with ACTA2, and purified by antigen affinity chromatography.
 
来源Monoclonal Mouse IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
浓度Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:


Figure1.Application in WB
Western blot analysis of extracts of various cells lines and tissues, using ACTA2 antibody CM00526HuM10-A1T at 1μg/mL. Secondary antibody: HRP  Mouse Anti-Rabbit IgG (H+L) at 1:20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

 

Figure2.Application in IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon、human lung、monkey epididymis、monkey heart、monkey kidney、monkey liver、monkey rectum、monkey stomach、monkey tongue sections labelling ACTA2 with purified CM00526HuM10-A1T at 5μg/mL dilution. Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1:2000 dilution. PBS instead of the primary antibody was used as the negative control.



别称ACT-A2, ACTSA, ACTVS, Asma, GIG46, Cell growth-inhibiting gene 46 protein
 
背景信息ACTA2 (also known as 伪-smooth muscle actin or 伪-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.

全称Actin, aortic smooth muscle (ACTA2)