Anti-TUBB3 Antibody, Mouse Monoclonal, C1F

产品编号：CM01147HuM10-C1F
   
$ 询价

规格     100ul
 

产品名称：Anti-TUBB3 Antibody, Mouse Monoclonal, C1F
 
克隆号：C1F
 
抗体亚型：/
 
经验证的应用：/
 
交叉反应：Predict reacts with: Human/Mouse/Rat
 
特异性：/

免疫原：
 
制备方法：Produced in mouse immunized with TUBB3, and purified by antigen affinity chromatography.
 
来源：Monoclonal Mouse IgG
 
纯化：Immunogen affinity purified
 
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物：Unconjugated
 
浓度：Liquid
 
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片：






Figure1.Western blot analysis of extracts of various lane1 Mouse brain(+++)、lane2  Rat brain(+++), using TUBB3 antibody (CM01147HuM10-C1F) at 2ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1：20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1.5min.











 


Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse brain sections labelling TUBB3 with purified CM01147HuM10-C1F at 10ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1：2000 dilution. PBS instead of the primary antibody was used as the negative control. 


 

别称：MC1R, TUBB4, Tubulin beta-4 chain
 
背景信息：There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.

全称：Tubulin beta-3 chain (TUBB3)
 
说明书：待上传