Anti-Ubiquitin Antibody,  Recombinant Antibody,  R1

产品编号:RPTMA1M1-R1
   
¥ 
询价

规格     100ug

说明书
产品名称Anti-Ubiquitin Antibody,  Recombinant Antibody,  R1
 
克隆号/
 
抗体亚型/
 
经验证的应用WB/IHC
 
交叉反应all
 
特异性Detect ubiquitin, polyubiquitin and ubiquitin protein and recombinant NEDD8

免疫原Recombinant full length protein corresponding to Bovine Ubiquitin
 
制备方法/
 
来源Recombinant Mouse IgG
 
纯化Protein A purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
状态:Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:








Figure1.Western blot analysis of 293T, HeLa and HepG2 cells, untreated or treated with the  MG132 (50 µM, 90 minutes), using RPTMA1M1-R1  at 2ug/ml dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L)  at 1:5000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit . Loading control to GAPDH (CP00002HuA10).









Figure2.Immunofluorescent analysis of Hela cells , using RPTMA1M1-R1 (green) at 10ug/ml dilution. Nuclear DNA was labelled in blue with DAPI (blue).  





 
别称/
 
背景信息Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue. The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as IκB, p53, cdc25A, and Bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis.

全称/