Anti-H2B (Lys120)-Ubi antibody, Rabbit Monoclonal

产品编号：RPTM006A1
   
￥ 询价

规格     100ug

浓度     1.3mg/ml
 

产品名称：Anti-H2B (Lys120)-Ubi antibody, Rabbit Monoclonal
 
克隆号：R1
 
抗体亚型：Rabbit IgG
 
经验证的应用：WB/ChIP/IF
 
交叉反应：Human, Mouse
 
特异性：Ubiquityl-Histone H2B (Lys120) (D11) XP® Rabbit mAb can recognize endogenous levels of histone H2B only when histone H2B is ubiquitinated at Lys120. The antibody does not cross-react with other ubiquitinated proteins or free ubiquitin.

免疫原：Synthetic peptide
 
宿主：CHOS
 
来源：Recombinant Rabbit IgG
 
纯化：Protein A purified
 
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
状态：Liquid
 
应用：The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.       WB: 0.5~5ug/ml          IF: 5~20ug/ml        ChIP:2-20ug/5×106cells
 
使用&储存条件：Shipped at 4℃.Store at 4℃ for frequent use.Aliquot and store at -20℃ for 12 months.Avoid repeated freezing/thawing and violent shaking.Please centrifuge it, before using.

质量验证图：


Figure1.Application in WB
Western blot analysis of extracts of  cell lines, using H2B (Lys120)-Ubi antibody (RPTM006A1) at 2ug/ml dilution. Secondary antibody: HRP Goat Anti-Rabbit  IgG (H+L)  at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. 












Figure2. Application in ICC（Hela）
Immunocytochemistry analysis of  Hela (Human epithelial cell line from cervix adenocarcinoma) cells labeling H2B (Lys120)-Ubi with purified RPTM006A1 at 10 µg/mL dilution.Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.2% tritonX-100. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.






Figure1.Application in ChIP
Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Ubiquityl-Histone H2B (Lys120) (D11) XP® Rabbit mAb or Normal Rabbit IgG using RPTM006A1. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human γ-Actin Promoter Primers, SimpleChIP® Human γ-Actin Intron 3 Primers, SimpleChIP® Human GAPDH Promoter Primers, and SimpleChIP® Human GAPDH Intron 2 Primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.


别称：/
 
背景信息：The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of posttranslational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Ubiquitin is a conserved 76 amino acid peptide unit that can be covalently linked to many cellular proteins by the ubiquitination process. Three components are involved in this protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thioester complex with the activation component E1; the activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue. Histone H2A is mono-ubiquitinated at Lys119 by the Polycomb Repressor Complex 1 (PRC1) and is critical for transcriptional silencing of the developmental HOX genes and X chromosome inactivation. PRC1 is composed of Bmi1 and RING1A (also RING1 or RNF1), both of which act to enhance the E3 ubiquitin ligase activity of the catalytic subunit RING1B (also RING2 or RNF2). Histone H2A is also mono-ubiquitinated at Lys119 at sites of DNA damage. This mono-ubiquitination event requires the PRC1 components Bmi1 and RING1B, in addition to another E3 ubiquitin ligase RNF8, and contributes to subsequent recruitment of the BRCA1 complex, via binding of RAP80/UIMC1 (ubiquitin interactive motif containing 1 protein).

全称：/
 
说明书：待上传