Anti-m6A antibody, Rabbit Monoclonal

产品编号:RPTM021A1
   
¥ 
询价

规格     100ug

浓度     1.3mg/ml
 

产品名称Anti-m6A antibody, Rabbit Monoclonal
 
克隆号R1
 
抗体亚型Rabbit IgG
 
经验证的应用Dot blot or RNA-IP-Seq
 
交叉反应All
 
特异性Only specifically recognize m6A modified epitopes.

免疫原Synthetic peptide
 
宿主CHOS
 
来源Recombinant Rabbit IgG
 
纯化Protein A purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
状态:Liquid
 
应用The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.       Dot blot:1:500-1:2000 
 
使用&储存条件Shipped at 4℃.Store at 4℃ for frequent use.Aliquot and store at -20℃ for 12 months.Avoid repeated freezing/thawing and violent shaking.Please centrifuge it, before using.

质量验证图:






Figure1.Specificity of RPTM021A1 was determined by ELISA. The antibody was titrated against an RNA oligo containing either unmodified adenosine or N6-methylated adenosine (m6A). As shown in the graph, the antibody only binds to N6-methylated adenosine (m6A).











Figure2.Specificity of RPTM021A1 was determined by competitive ELISA. The graph depicts the binding of the antibody to a pre-coated m6A oligonucleotide in the presence of increasing concentrations of differentially modified adenosine. As shown in the graph, antibody binding is only blocked by free m6A nucleoside.








别称m6A, N6-methyladenosine
 
背景信息N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment.

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