Anti-m6A antibody, Rabbit Monoclonal
产品编号:RPTM021A1
¥ 询价
规格 100ug
浓度 1.3mg/ml
产品名称:Anti-m6A antibody, Rabbit Monoclonal
克隆号:R1
抗体亚型:Rabbit IgG
经验证的应用:Dot blot or RNA-IP-Seq
交叉反应:All
特异性:Only specifically recognize m6A modified epitopes.
免疫原:Synthetic peptide
宿主:CHOS
来源:Recombinant Rabbit IgG
纯化:Protein A purified
缓冲液:Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
状态:Liquid
应用:The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Dot blot:1:500-1:2000
使用&储存条件:Shipped at 4℃.Store at 4℃ for frequent use.Aliquot and store at -20℃ for 12 months.Avoid repeated freezing/thawing and violent shaking.Please centrifuge it, before using.
质量验证图:
Figure1.Specificity of RPTM021A1 was determined by ELISA. The antibody was titrated against an RNA oligo containing either unmodified adenosine or N6-methylated adenosine (m6A). As shown in the graph, the antibody only binds to N6-methylated adenosine (m6A).
Figure2.Specificity of RPTM021A1 was determined by competitive ELISA. The graph depicts the binding of the antibody to a pre-coated m6A oligonucleotide in the presence of increasing concentrations of differentially modified adenosine. As shown in the graph, antibody binding is only blocked by free m6A nucleoside.
别称:m6A, N6-methyladenosine
背景信息:N6-Methyladenosine (m6A) is an abundant modification found in mRNA, tRNA, snRNA, as well as long non-coding RNA, in all species. RNA adenosine methylation is catalyzed by a multicomponent complex composed of METTL3/MT-A70, METTL14, and WTAP in mammals. METTL3 & METTL14 are responsible for the methyltransferase activity of the complex, and WTAP mediates substrate recruitment.
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