Anti-crotonyllysine Antibody, Recombinant Antibody

产品编号:RPTMC9M1
   
¥ 
询价

规格     100ug

浓度     1.3mg/ml
 

产品名称Anti-crotonyllysine antibody, Mouse Monoclonal
 
克隆号R1
 
抗体亚型Mouse IgG1
 
经验证的应用WB
 
交叉反应Mouse
 
特异性Only specifically recognize crotonyllysine modified epitopes.

免疫原Synthetic peptide
 
宿主CHOS
 
来源Recombinant Mouse IgG
 
纯化Protein A purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
状态:Liquid
 
应用The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.       WB: 0.5~5ug/ml
 
使用&储存条件Shipped at 4℃.Store at 4℃ for frequent use.Aliquot and store at -20℃ for 12 months.Avoid repeated freezing/thawing and violent shaking.Please centrifuge it, before using.

质量验证图:



Figure1.Application in WB
Western blot analysis of extracts of  cell lines or tissues, using crotonyllysine antibody (RPTMC9M1) at 2ug/ml dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. 












Figure2.Application in ICCHela
Immunocytochemistry analysis of  Hela (Human epithelial cell line from cervix adenocarcinoma) cells labeling crotonyllysine with purified RPTMC9M1 at 10 µg/mL dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.2% tritonX-100. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.


别称Kcr, 巴豆酰化
 
背景信息/

全称/
 
说明书待上传