Anti-nitrotyrosine antibody, Mouse Monoclonal

产品编号:RPTM032M1
   
¥ 
询价

规格     100ug

浓度     1.3mg/ml
 

产品名称Anti-nitrotyrosine antibody, Mouse Monoclonal
 
克隆号R1
 
抗体亚型Mouse IgG1
 
经验证的应用WB
 
交叉反应All
 
特异性Only specifically recognize nitrotyrosine modified epitopes.

免疫原Synthetic peptide
 
宿主CHOS
 
来源Recombinant Rabbit IgG
 
纯化Protein A purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
状态:Liquid
 
应用The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.       WB: 0.5~5ug/ml  
 
使用&储存条件Shipped at 4℃.Store at 4℃ for frequent use.Aliquot and store at -20℃ for 12 months.Avoid repeated freezing/thawing and violent shaking.Please centrifuge it, before using.

质量验证图:





Figure1.Application in WB
Western blot analysis of extracts of  cell lines (treated with or with not 3mM peroxynitrite for 30 mins), using nitrotyrosine antibody (RPTM032M1) at 2ug/ml dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Blocking buffer: 5% nonfat dry milk in TBST. 






 
别称nitro-Tyr, Nitro-Tyrosine
 
背景信息Nitric oxide (NO) is implicated in carcinogenesis, chronic infection, inflammation and neurodegeneration. High levels of both superoxide and nitric oxide in tissues interact to form peroxynitrite, a potent oxidant that can modify Tyr residues in proteins to form 3-nitro-tyrosine. Tyrosine nitration of mitochondrial manganese superoxide dismutase results in loss of enzymatic activity. The nitration of p53 at Tyr residues abolishes its capacity for binding to its DNA consensus sequence.

全称/
 
说明书待上传