Anti-KRT19 Antibody, Mouse Monoclonal, F5T

产品编号:MA00104HuM10-F5T
   
询价

规格     100ul

说明书

产品名称Anti-KRT19 Antibody, Mouse Monoclonal, F5T
 
克隆号F5T
 
抗体亚型:/
 
经验证的应用/
 
交叉反应Predict reacts with: Human/Mouse/Rat
 
特异性:/

免疫原A synthetic peptide of human KRT19
 
制备方法Produced in mouse immunized with KRT19, and purified by antigen affinity chromatography.
 
来源Monoclonal Mouse IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
浓度Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:






Figure1.Western blot analysis of extracts of various lane1 human placenta、lane2 MCF7、lane3 rat placenta, using KRT19 antibody (MA00104HuM10-F5T) at 1ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5min.











 


Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Monkey epididymis、Monkey jejunum、Monkey kidney、Monkey liver、Monkey prostate、Monkey submandibular gland sections labelling KRT19 with purified MA00104HuM10-F5T at 5ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1:2000 dilution. PBS instead of the primary antibody was used as the negative control.


 
别称CK 19, CK19, Cytokeratin 19, K19, K1CS, keratin 19, KRT19
 
背景信息Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells. Keratin expression is highly regulated, tissue specific, and varies according to cell-state. Type I keratins consist of acidic, low molecular weight proteins with MW ranging from 40 kDa (KRT19) to 64 kDa (KRT9). Type 2 keratins consist of basic or neutral, high molecular weight proteins  with MW from 52 kDa (KRT8) to 67 kDa (KRT18). Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

全称Keratin, type I cytoskeletal 19 (KRT19)