产品名称:Anti-PRPH Antibody, Mouse Monoclonal, H4T
克隆号:H4T
抗体亚型:/
经验证的应用:/
交叉反应:Predict reacts with: Human/Mouse/Rat
特异性:/
免疫原:Recombinant human PRPH Protein
制备方法:Produced in mouse immunized with PRPH, and purified by antigen affinity chromatography.
来源:Monoclonal Mouse IgG
纯化:Immunogen affinity purified
缓冲液:Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物:Unconjugated
浓度:Liquid
运输方式:This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片:
Figure1.Western blot analysis of extracts of various lane1 HepG2、lane2 SH-N-SH、lane3 mouse brain、lane4 rat brain, using PRPH antibody (MA00927HuM10-H4T) at 3ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 12min.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Monkey brain、Monkey cerebellum、Monkey esophagus sections labelling PRPH with purified MA00927HuM10-H4T at 10ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1:2000 dilution. PBS instead of the primary antibody was used as the negative control.
别称:NEF4, PRPH1, Neurofilament 4
背景信息:Peripherin is a neuron-specific intermediate filament protein that is particularly expressed in peripheral nervous system. It is used to study peripheral nerve development and regeneration, as it is highly expressed during elongation of axon. Peripherin is a routine marker for unmyelinated neurons in trigeminal ganglion (TG) and the dorsal root ganglion (DRG). Peripherin is also expressed in peri-insular areas of islets and it has been identified as a key autoantigen for type 1A diabetes.
全称:Peripherin (PRPH)