产品名称:Anti-SRC Antibody, Mouse Monoclonal, F3T
克隆号:F3T
抗体亚型:/
经验证的应用:/
交叉反应:Predict reacts with: Human/Mouse/Rat
特异性:/
免疫原:Recombinant human SRC protein
制备方法:Produced in mouse immunized with SRC, and purified by antigen affinity chromatography.
来源:Monoclonal Mouse IgG
纯化:Immunogen affinity purified
缓冲液:Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物:Unconjugated
浓度:Liquid
运输方式:This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片:
Figure1.Western blot analysis of extracts of various lane1 hela、lane2 293T、lane3 rat brain、lane4 mouse brain, using SRC antibody (MA15522HuM10-F3T) at 5µg/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1min.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Monkey kidney、Monkey testis、Monkey epididymis sections labelling SRC with purified MA15522HuM10-F3T at 30ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1:2000 dilution. PBS instead of the primary antibody was used as the negative control.
别称:ASV, c SRC, p60 Src, pp60c src, Proto oncogene c Src, SRC, SRC1
背景信息:SRC, also named as SRC1 and p60-Src, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor protein tyrosine kinase that plays pivotal roles in numerous cellular processes such as proliferation, migration, and transformation. In concert with PTK2B, SRC plays an important role in osteoclastic bone resorption. It promotes energy production in osteoclasts by activating mitochondrial cytochrome C oxidase. SRC can be detected as 60 kDa and 55 kDa (PMID: 15047935, 21461171).
全称:Proto-oncogene tyrosine-protein kinase Src (SRC)