产品名称：Anti-IL2RA Antibody, Mouse Monoclonal, H2T
克隆号：H2T
抗体亚型：/
经验证的应用：/
交叉反应：Predict reacts with: Human/Mouse/Rat
特异性：/
免疫原：A synthetic peptide of human IL2RA
制备方法：Produced in mouse immunized with IL2RA, and purified by antigen affinity chromatography.
来源：Monoclonal Mouse IgG
纯化：Immunogen affinity purified
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物：Unconjugated
浓度：Liquid
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片：
Figure1.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen、Monkey rectum sections labelling IL2RA with purified MA00596HuM10-H2T at 30ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1：2000 dilution. PBS instead of the primary antibody was used as the negative control.
别称：CD25, IL2R-a, IL2-RA, IDDM10, IL2R, TCGFR, p55, TAC antigen
背景信息：CD25/IL-2R alpha. IL-2receptor alpha (IL-2R alpha), also known as CD25, is a 55 kDa type I membraneglycoprotein that belongs to the family of cytokine receptors that utilize thecommon gamma chain subunit (gamma c). Human IL-2R alpha cDNAencodes a 213 amino acid (aa) precursor with a 21 aa signal peptide and a 192 aaextracellular region. The ECD of Human IL-2R alpha shares a 59% amino acid sequenceidentity with the ECD of mouse and rat IL-2R alpha, respectively. IL‑2R alpha is primarily expressed on activated Tcells and on regulatory T cells (Treg) (1-3). IL-2R beta (CD122) and gamma c (IL-2R gamma /CD132) dimerize to form a constitutively expressed intermediateaffinity IL-2 receptor (4, 5). By itself, IL-2R alpha binds IL-2 with lowaffinity. IL-2R alpha makes no contacts with IL-2R beta or gamma c,and only minor changes are observed in the IL-2 structure in response toreceptor binding. These findings support the principal role of IL-2R alpha todeliver IL-2 to the signaling complex and act as regulator of signaltransduction (6, 7). Asoluble form of IL‑2R alpha can be generated by proteolyticcleavage of the cell surface receptor, rendering the T cell unresponsive toIL-2 (8, 9). Increased serum levels of soluble IL‑2R alpha are found in some cancers and immunedisorders (10). IL-2R alpha is required for activation induced cell death(AICD) of naive T cells, a mechanism responsible for deleting autoreactive T cell clones (11, 12). IL-2R alpha is also required for the development ofCD4+CD25+ Treg which suppresses autoreactive CD4+ T cells, thereby contributingto peripheral T cell homeostasis (11-13).
全称：Interleukin-2 receptor subunit alpha (IL2RA)