Anti-GAP43 Antibody, Mouse Monoclonal, E4T

产品编号:MA00913HuM10-E4T
   
询价

规格     100ul

说明书

产品名称Anti-GAP43 Antibody, Mouse Monoclonal, E4T
 
克隆号E4T
 
抗体亚型:/
 
经验证的应用/
 
交叉反应Predict reacts with: Human/Mouse/Rat
 
特异性:/

免疫原Recombinant Human GAP43 protein
 
制备方法Produced in mouse immunized with GAP43, and purified by antigen affinity chromatography.
 
来源Monoclonal Mouse IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
浓度Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:






Figure1.Western blot analysis of extracts of various lane1 rat brain、lane2 mouse brain, using GAP43 antibody (MA00913HuM10-E4T) at 3ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1min.










 


Figure1.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Monkey brain、Monkey cerebellum、Monkey brain stem sections labelling GAP43 with purified MA00913HuM10-E4T at 10ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1:2000 dilution. PBS instead of the primary antibody was used as the negative control.


 

别称F1, B-50, PP46, Neuron Growth-Associated Protein 43, Neuromodulin, Axonal Membrane Protein GAP-43, Protein F1, Calmodulin-Binding Protein P-57, Neural Phosphoprotein B-50
 
背景信息The neuronal growth-associated protein GAP43 is also known as neuromodulin, B-50, P-57, F1 and pp46. Deficiency of GAP43 in mice results in death early in the postnatal period. GAP43 is one of the main substrates for protein kinase C in the brain. GAP43 is an intracellular growth-associated protein that appears to assist neuronal pathfinding and branching during development and regeneration, and may contribute to presynaptic membrane changes in the adult, leading to the neurotransmitter release, endocytosis and synaptic vesicle recycling, long-term potentiation, spatial memory formation, and learning. The predicated molecular weight of about 25 kDa is much lower than the apparent observed molecular weight of 43 kDa on SDS-PAGE gels, and this occurs because the highly charged nature of GAP43 causes it to bind less than the average amount of SDS per amino acid, and because the protein has an elongated structure.

全称Neuromodulin (GAP43)
 
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