Anti-MICU1 Antibody, Mouse Monoclonal, E4T

产品编号:MA04948HuM10-E4T
   
询价

规格     100ul

说明书
 

 

产品名称Anti-MICU1 Antibody, Mouse Monoclonal, E4T
 
克隆号E4T
 
抗体亚型:/
 
经验证的应用/
 
交叉反应Predict reacts with: Human/Mouse/Rat
 
特异性:/

免疫原Recombinant Human MICU1 protein
 
制备方法Produced in mouse immunized with MICU1, and purified by antigen affinity chromatography.
 
来源Monoclonal Mouse IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
浓度Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:






Figure1.Western blot analysis of extracts of various lane1 MCF7、lane2 mouse brain、lane3 rat brain, using MICU1 antibody (MA04948HuM10-E4T) at 5ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 7min.











 


Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Monkey brain stem sections labelling MICU1 with purified MA04948HuM10-E4T at 30ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1:2000 dilution. PBS instead of the primary antibody was used as the negative control.


 



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