Anti-PTEN Antibody, Mouse Monoclonal, C1T

产品编号:MA00287HuM10-C1T
   
询价

规格     100ul

说明书

产品名称Anti-PTEN Antibody, Mouse Monoclonal, C1T
 
克隆号C1T
 
抗体亚型:/
 
经验证的应用/
 
交叉反应Predict reacts with: Human/Mouse/Rat
 
特异性:/

免疫原A synthetic peptide of human PTEN
 
制备方法Produced in mouse immunized with PTEN, and purified by antigen affinity chromatography.
 
来源Monoclonal Mouse IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
浓度Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:


Figure1.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Monkey cerebellum、Monkey kidney、Monkey prostate、Monkey epididymis sections labelling PTEN with purified MA00287HuM10-C1T at 30ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1:2000 dilution. PBS instead of the primary antibody was used as the negative control.


 

别称BZS, MHAM, MMAC1, PTEN1, TEP1, Mutated In Multiple Advanced Cancers 1, Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
 
背景信息PTEN. The tumor suppressor gene PTEN (phosphatase and tensin homolog deleted on chromosome 10), also known as MMAC1 (mutated in multiple advanced cancers 1), encodes a phosphatase that contains the catalytic signature motif (HCXXGXXRS/T) found in all members of the protein tyrosine phosphatase family. In vitro, the recombinant PTEN has both lipid phosphatase and protein phosphatase activities (1, 2). Interestingly, accumulating evidence has shown that the tumor suppressor activity of PTEN relies on its ability to dephosphorylate phosphatidylinositol (3, 4, 5)-triphosphate specifically at position 3 of the inositol ring (3). This activity reduces the levels of phosphatidylinositol (3, 4, 5)-triphosphate which is specifically produced from phosphatidylinositol (4, 5)-diphosphate by PI 3-kinase upon activation by a variety of stimuli. Therefore, PTEN antagonizes PI 3-kinase-induced downstream signaling events and cellular processes including cell growth, apoptosis and cell motility. In vivo, the importance of PTEN catalytic activity in its tumor suppressor functions is underscored by the fact that the majority of PTEN missense mutations detected in tumor specimens target the phosphatase domain and cause a loss in PTEN phosphatase activity (4).

全称Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (PTEN)