Anti-TGFB1 Antibody, Mouse Monoclonal, H7F

产品编号:MA00047HuM20-H7F
   
询价

规格     100ul

说明书

产品名称Anti-TGFB1 Antibody, Mouse Monoclonal, H7F
 
克隆号H7F
 
抗体亚型:/
 
经验证的应用/
 
交叉反应Predict reacts with: Human/Mouse/Rat
 
特异性:/

免疫原:/
 
制备方法Produced in mouse immunized with TGFB1, and purified by antigen affinity chromatography.
 
来源Monoclonal Mouse IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
浓度Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:






Figure1.Western blot analysis of extracts of various lane1 NIH/3T3(+)、lane2 K-562(+), using TGFB1 antibody (MA00047HuM20-H7F) at 5ug/ml Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1.5min.











 


Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse thyroid cancer sections labelling TGFB1 with purified MA00047HuM20-H7F at 30ug/ml dilution . Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1:2000 dilution. PBS instead of the primary antibody was used as the negative control. 


 
别称TGF-B1, CED, DPD1, LAP, Camurati-Engelmann Disease, Latency-associated peptide
 
背景信息Transforming Growth Factor Beta 1, 2, and 3 (TGF-beta 1, TGF-beta 2, and TGF-beta 3) are highly pleiotropic cytokines that virtually all cell types secrete. TGF-beta molecules are proposed to act as cellular switches that regulate processes such as immune function, proliferation, and epithelial-mesenchymal transition. Targeted deletions of these genes in mice show that each TGF-beta isoform has some non-redundant functions: TGF-beta 1 is involved in hematopoiesis and endothelial differentiation; TGF-beta 2 affects development of cardiac, lung, craniofacial, limb, eye, ear, and urogenital systems; and TGF-beta 3 influences palatogenesis and pulmonary development. The full range of in vitro biological activities of TGF-beta 5 has not yet been explored. However, TGF-beta 1, TGF-beta 2, TGF-beta 3, and TGF-beta 5 have been found to be largely interchangeable in an inhibitory bioassay, and it is anticipated that TGF-beta 5 will show a spectrum of activities similar to the other TGF-beta family members. To date, the production of TGF-beta 5 has only been demonstrated in Xenopus.

全称Transforming growth factor beta-1 proprotein (TGFB1)