Anti-CASP3 Antibody, Mouse Monoclonal, C2

产品编号:PM00067HuM10-C2
  
询价

规格     50uL  100uL  200uL   可选

产品名称Anti-CASP3 Antibody, Mouse Monoclonal, C2

克隆号:C2
 
抗体亚型
 
经验证的应用WB/ICC
 
交叉反应
 
特异性human CASP3

免疫原Recombinant human CASP3 protein, fragment Ser29~Asp175; UniprotKB: p42574
 
制备方法Produced in rabbits immunized with human CASP3, and purified by antigen affinity chromatography.
 
来源Monoclonal Mouse IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
浓度Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

别称CPP32, CPP32B, SCA1, Apoptain, Yama, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 3, SREBP cleavage activity 1
 
背景信息Caspase-3. Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa member of the peptidase C14A family of enzymes (1, 2, 3). It is widely expressed and is an integral component of the apoptotic cascade. Caspase-3 is considered to be the major executioner caspase; that is, the primary downstream mediator of apoptotic-associated proteolysis (2, 3, 4). Active Caspase-3 is known to utilize a Cys residue to cleave multiple substrates, including PARP, proIL‑16, PKC-gamma  & -δ, procaspases 6, 7 and 9, and  beta ‑catenin (1). Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein (5, 6, 7). Normally, it is an inactive, cytosolic homodimer, but following an upstream signal that activates processing proteases, procaspase-3 undergoes proteolytic cleavage (1, 2, 8, 9). This generates an N-terminal 175 aa p20/20 kDa subunit plus a 102 aa C-terminal p12/12 kDa subunit, followed by further processing of the p20 subunit at Asp28 to generate a final p17 subunit (aa 29‑175) (9). The p17 and p12 subunits noncovalently heterodimerize, and subsequently associate with another p17/p12 heterodimer to form an active antiparallel homodimer. The p17 subunit contains the enzyme active site (aa 161‑165), with an embedded catalytic Cys which is normally nitrosylated and inactive. Full activation requires both proteolytic processing and Cys163 denitrosylation (10). Multiple proteases can use Caspase-3 as a substrate including Caspase-6, -8, and -10, granzyme B, and Caspase-3 itself (9, 11, 12, 13).

全称Caspase-3 (CASP3)
 
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