Anti-NQO1 Antibody, Mouse Monoclonal, C6

产品编号：PM00367HuM10-C6
   
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规格     50uL  100uL  200uL   可选

产品名称：Anti-NQO1 Antibody, Mouse Monoclonal, C6
 
克隆号：C6
 
抗体亚型：
 
经验证的应用：IHC/IF/ICC
 
交叉反应：
 
特异性：human NQO1

免疫原：Recombinant human NQO1 protein, fragment Met1~Lys274; UniprotKB: P15559
 
制备方法：Produced in rabbits immunized with human NQO1, and purified by antigen affinity chromatography.
 
来源：Monoclonal Mouse IgG
 
纯化：Immunogen affinity purified
 
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物：Unconjugated
 
浓度：Liquid
 
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片：


Figure1.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human  breast cancer  sections labelling NQO1 with purified PM00367HuM10-C6 at 10ug/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0).  Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.




 



Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human  rectal cancer  sections labelling NQO1 with purified PM00367HuM10-C6 at 10ug/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0).  Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.



 
 
别称：DHQU, DIA4, DTD, NMOR1, NMORI, QR1, Azoreductase, DT-diaphorase, Menadione reductase, Phylloquinone reductase, Quinone reductase 1, NAD(P)H:quinone oxidoreductase 1
 
背景信息：NQO-1. NAD(P)H:quinone acceptor oxidoreductase 1 (NQO1), also known as DT-diaphorase, is a widely-distributed FAD-dependent flavoprotein that promotes 2-electron reductions of quinones, quinoneimines, nitroaromatics, and azo dyes (1, 2). As a result it prevents the one electron reduction of quinones that results in the production of radical species. NQO1 is a highly-inducible enzyme that is regulated by the Keap1/Nrf2/ARE pathway (2, 3). The increase and decrease of NQO1 levels are associated with decreased and increased susceptibilities to oxidative stress, respectively. Thus, NQO1 is a marker cytoprotective enzyme in oxidative stress. Independently of its catalytic function, NQO1 plays a role in regulating the proteosomal degradation of p53, p73a, and p33 (2). NQO1 physically interacts with p53 and p73 in an NADH-dependent manner and protects them from 20S proteasomal degradation in a ubiquitin independent pathway (4).
 
全称：NAD (P)H dehydrogenase (NQO1)
 
说明书：待上传