Anti-GAPDH Antibody, Rabbit Polyclonal
产品编号：CP00002HuA10
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产品名称：Anti-GAPDH Antibody, Rabbit Polyclonal
经验证的应用：WB/IHC/ICC
交叉反应：Predict reacts with: Human/Mouse/Rat
特异性：human GAPDH
免疫原：Recombinant human GAPDH protein, fragment Gly2~Glu335; UniprotKB: P04406
制备方法：Produced in rabbits immunized with human GAPDH, and purified by antigen affinity chromatography.
来源：Polyclonal Rabbit IgG
纯化：Immunogen affinity purified
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物：Unconjugated
状态：Liquid
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片：
Figure1.Application in WB
Figure2.Application in WB
Western blot analysis of extracts of various cell lines using GAPDH antibody (CP00002HuA10) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s..
Figure3.Application in ICC
Immunocytochemistry analysis of Hela(Human cervical cancer cell) cells labeling GAPDH with purified CP00002HuA10 at 5 µg/mL. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Goat anti-Rabbit IgG (Alexa Fluor® 488, GB25303) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Figure4.Application in IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue sections labelling GAPDH with purified CP00002HuA10 at 5ug/ml. Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
背景信息：Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
全称：Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)