Anti-ACTA2 Antibody, Rabbit Polyclonal

产品编号：CP00526HuA10
   
$ 询价

规格     50uL  100uL  200uL  可选

产品名称：Anti-ACTA2 Antibody, Rabbit Polyclonal
 
经验证的应用：WB/ICC
 
交叉反应：/
 
特异性：human ACTA2
 
免疫原：A synthetic peptide of  human ACTA2; UniprotKB: P62736
 
制备方法：Produced in rabbits immunized with human ACTA2, and purified by antigen affinity chromatography
 
来源：Polyclonal Rabbit IgG
 
纯化：Immunogen affinity purified
 
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物：Unconjugated
 
状态：Liquid
 
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片：






Figure1.Western blot analysis of extracts of various cell lines  using ACTA2 antibody (CP00526HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 8s.








 
 
























Figure2.Western blot analysis of extracts of various cell lines  using ACTA2 antibody (CP00526HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 8s.






















 


Figure3.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine  sections labelling ACTA2 with purified CP00526HuA10 at 5ug/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0).  Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.











 



Figure4.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas   sections labelling ACTA2 with purified CP00526HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0).  Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.









 

别称：ACT-A2, ACTSA, ACTVS, Asma, GIG46, Cell growth-inhibiting gene 46 protein
 
背景信息：ACTA2 (also known as 伪-smooth muscle actin or 伪-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins
 
全称：Actin, aortic smooth muscle (ACTA2)
 
说明书：
说明书