Anti-HSPA1A Antibody, Rabbit Polyclonal
产品编号：CP00175HuA10
$ 询价
规格 50uL 100uL 200uL 可选
产品名称：Anti-HSPA1A Antibody, Rabbit Polyclonal
经验证的应用： WB/ICC
交叉反应：/
特异性：human HSPA1A
免疫原：Recombinant human HSPA1A protein, fragment Ala2~Asp641; UniprotKB: P0DMV8
制备方法：Produced in rabbits immunized with human HSPA1A, and purified by antigen affinity chromatography.
来源：Polyclonal Rabbit IgG
纯化：Immunogen affinity purified
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物：Unconjugated
状态：Liquid
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片：
Figure1.Western blot analysis of extracts of various cell lines using HSPA1A antibody (CP00175HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 1.5s.
Figure2.Western blot analysis of extracts of various cell lines using HSPA1A antibody (CP00175HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 0.5s.
Figure3.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin sections labelling KDR with purified CP00175HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
Figure4.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney sections labelling KDR with purified CP00175HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
背景信息：HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol/nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is a useful marker for exosome. This antibody well recognized the endogenous HSP70 protein in multiple cell lines. (21373891)
全称：Heat shock 70 kDa protein 1A (Hspa1a)
说明书：待上传