Anti-ACTN1+ACTN2+ACTN3+ACTN4 Antibody, Rabbit Polyclonal
产品编号：PA00060Hu&00478HuA10
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规格 50uL 100uL 200uL
产品名称：Anti-ACTN1+ACTN2+ACTN3+ACTN4 Antibody, Rabbit Polyclonal
经验证的应用：WB
交叉反应：Predict reacts with: Human/Mouse/Rat
特异性：human ACTN1
免疫原：A synthetic peptide of human ACTN1+ACTN2+ACTN3+ACTN4; UniprotKB: P12814&P35609&Q08043&O43707
制备方法：Produced in rabbits immunized with human ACTN1, and purified by antigen affinity chromatography.
来源：Polyclonal Rabbit IgG
纯化：Immunogen affinity purified
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物：Unconjugated
状态：Liquid
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片：
Figure1.Western blot analysis of extracts of various cell lines and tissues , using ACTN1 antibody (PA00060Hu&00478HuA10) at 2ug/ml dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Blocking buffer: 5% nonfat dry milk in TBST.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart sections labelling ACTN1+ACTN2+ACTN3+ACTN4 with purified PA00060Hu&00478HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution (EDTA, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
别称：Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1
背景信息：Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.
全称：Alpha-actinin-1 (ACTN1)
说明书：待上传
经验证的应用：WB
交叉反应：Predict reacts with: Human/Mouse/Rat
特异性：human ACTN1
免疫原：A synthetic peptide of human ACTN1+ACTN2+ACTN3+ACTN4; UniprotKB: P12814&P35609&Q08043&O43707
制备方法：Produced in rabbits immunized with human ACTN1, and purified by antigen affinity chromatography.
来源：Polyclonal Rabbit IgG
纯化：Immunogen affinity purified
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物：Unconjugated
状态：Liquid
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片：
Figure1.Western blot analysis of extracts of various cell lines and tissues , using ACTN1 antibody (PA00060Hu&00478HuA10) at 2ug/ml dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Blocking buffer: 5% nonfat dry milk in TBST.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart sections labelling ACTN1+ACTN2+ACTN3+ACTN4 with purified PA00060Hu&00478HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution (EDTA, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
别称：Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1
背景信息：Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.
全称：Alpha-actinin-1 (ACTN1)
说明书：待上传