Anti-F11 Antibody, Rabbit Polyclonal

产品编号:PA00206HuA10
   
询价

规格     50uL  100uL  200uL  可选
产品名称Anti-F11 Antibody, Rabbit Polyclonal
 
经验证的应用:WB/IHC

交叉反应Hu/Mu/Ra
 
特异性human F11
 
免疫原Recombinant human F11 protein, fragment Glu19~Arg387; UniprotKB: P03951

制备方法Produced in rabbits immunized with human F11, and purified by antigen affinity chromatography.
 
来源Polyclonal Rabbit IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
状态Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:








Figure1.Western blot analysis of extracts of various cell lines and tissue, using F11 antibody (PA00206HuA10) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)  at 1:10000 dilution.

























 




Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human  liver sections labelling F11 with purified PA00206HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using  Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution  (EDTA, pH 9.0).  Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.















 


Figure3.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human  small intestine sections labelling F11 with purified PA00206HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using  Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution  (EDTA, pH 9.0).  Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.

 













别称FXI, PTA, Fletcher Factor, Prekallikrein, Plasma Thromboplastin Antecedent

背景信息Coagulation Factor XI. Coagulation factors XI and XIa refer to the pro and active forms of the same protease, respectively (1). Factor XI is synthesized in the liver and circulates in the plasma as a disulfide bond-linked dimer complexed with high molecular weight kininogen. Factor XI is converted into XIa via either the contact phase of blood coagulation or thrombin-mediated activation on the platelet surface. The resulting XIa converts factor IX into IXa, which subsequently activates factor X into Xa. Factor Xa in turn activates factor II/thrombin to complete the coagulation cascade. Patients with factor XI deficiency are prone to excessive bleeding after hemostatic challenge. There are two alternative splicing forms. Isoform 1 corresponds to the circulating plasma factor XI and isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells (2). The 625 amino acid precursor of isoform 1 consists of a signal peptide (residues 1 to 18) and the mature chain (residues 19 to 625). The mature chain (XI) can be further processed into the heavy chain (residues 19 to 387) and the light chain (residues 388 to 625) (XIa). The purified rhFactor XI corresponds to isoform 1 (residues 19 to 625), which can be activated by treatment with thermolysin under the conditions described in the Activity Assay Protocol.

全称Coagulation factor XI (F11)
 
说明书
说明书