Anti-CKM Antibody, Rabbit Polyclonal

产品编号:PA00011HuA10
   
询价

规格     50uL  100uL  200uL


说明书
产品名称Anti-CKM Antibody, Rabbit Polyclonal

经验证的应用WB
 
交叉反应Predict reacts with: Human/Mouse/Rat
 
特异性human CKM
 
免疫原Recombinant human CKM protein, fragment Met1~Lys381; UniprotKB: P06732
 
制备方法Produced in rabbits immunized with human CKM, and purified by antigen affinity chromatography.
 
来源Polyclonal Rabbit IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
状态Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:






Figure1.Application in WB
Western blot analysis of extracts of various tissues, using CKM antibody (PA00011HuA10) at 1 µg/mL dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0.8s.





别称CK-MM, CKMM, CK3, CK-M, MM-CK, M-CK, Creatine kinase M-type, N-terminally processed
 
背景信息Creatine Kinase MM/CKMM. Creatine kinase (CK) catalyzes the conversion of creatine to phosphocreatine (PCr) by consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP). CK reaction is reversible and thus ATP can be generated from PCr and ADP (1). In tissues and cells that consume ATP rapidly, especially skeletal muscle, but also brain, photoreceptor cells of the retina, hair cells of the inner ear, spermatozoa and smooth muscle, PCr serves as an energy reservoir for the rapid buffering and regeneration of ATP in situ, as well as for intracellular energy transport by the PCr shuttle or circuit (2). Clinically, creatine kinase is assayed in blood tests as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, autoimmune myositides and acute renal failure. Creatine kinase M (CKM), especially, is a cytoplasmic enzyme and is an important serum marker for myocardial infarction. It acts as a homodimer (MM type) in striated muscle as well as in other tissues, and forms a heterodimer (MB type) with CKB, a brain isozyme, in the heart (3). The recombinant human CKM is in the MM type and its activity is measured using a phosphatase-coupled method (4).
 
全称Creatine kinase M-type (CKM)