Anti-GALNT4 Antibody, Rabbit Polyclonal
产品编号：PA00561HuA10
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规格 50uL 100uL 200uL 可选
产品名称：Anti-GALNT4 Antibody, Rabbit Polyclonal
经验证的应用：WB
交叉反应：/
特异性：human GALNT4
免疫原：Recombinant human GALNT4 protein, fragment Ala36~Lys578; UniprotKB: Q8N4A0
制备方法：Produced in rabbits immunized with human GALNT4, and purified by antigen affinity chromatography
来源：Polyclonal Rabbit IgG
纯化：Immunogen affinity purified
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物：Unconjugated
状态：Liquid
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片：
Figure1.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil sections labelling GALNT4 with purified PA00561HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using EDTA buffer (pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
别称：pp-GaNTase 4
背景信息：Polypeptide GalNAc Transferase 4/GALNT4. O-glycosylation is a ubiquitous post-translational modification present in secreted and membrane-bound proteins. Polypeptide N-acetylgalactosaminyltransferases (GALNTs) calalyze the initial step for O-glycosylation by transferring GalNAc to Thr or Ser residues (GalNAc alpha 1-O-Ser/Thr) in the Golgi compartment. Structurally, the GALNTs consist of an N-terminal catalytic domain tethered by a short linker to a C-terminal ricin-like lectin domain containing three potential carbohydrate-binding sites (1, 2). Twenty distinct GALNT isoforms have been detected in humans. These isoforms display both unique and overlapping substrate specificities (3, 4, 5) with no known universal consensus glycosylation sequence. Glycosylation of mucins results from the successive, often hierarchical, action of several specific GALNTs (6). Human GALNT4 is widely expressed with particularly high expression in the immune system (7). The enzymatic specificity of GALNT4 depends on the selective binding between the C-terminal lectin domain and the existing O-GalNAc modified residues present within the substrate polypeptide (8). Along with GALNT3, GALNT4 is classified as an intermediate transferase that glycosylates mucin polypeptides that have been previously mono- or di-glycosylated by other transferases (6). The enzymatic activity of recombinant human GALNT4 was determined using a phosphatase-coupled assay (9)
全称：Polypeptide N-acetylgalactosaminyltransferase 4 (GALNT4)
说明书：待上传