Anti-CASP8 Antibody, Rabbit Polyclonal

产品编号：PA00277HuA30
   
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规格     50uL  100uL  200uL  可选

产品名称：Anti-CASP8 Antibody, Rabbit Polyclonal
 
经验证的应用：/

交叉反应：/
 
特异性：human CASP8

免疫原：Recombinant human CASP8 protein, fragment Met1~Glu195; UniprotKB: Q14790

制备方法：Produced in rabbits immunized with human CASP8, and purified by antigen affinity chromatography.

来源：Polyclonal Rabbit IgG
 
纯化：Immunogen affinity purified
 
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物：Unconjugated
 
状态：Liquid
 
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
 
别称：CAP4, FLICE, MACH, MCH5, Cysteinyl Aspartate Specific Proteinases 8, Apoptotic cysteine protease, FADD-homologous ICE/ced-3-like protease, MORT1-associated ced-3 homolog

背景信息：Caspase-8. Caspase-8 (Cysteine-aspartic acid protease 8/Casp8a; also named MCH5, FLICA and MACH alpha 1) is a 28 kDa member of the peptidase C14A family of enzymes (1, 2, 3). It is widely expressed and is considered an initiating caspase for the apoptotic cascade (4). Caspase-8 acts on a wide variety of substrates, including procaspases‑3, 4, 6, 7, 9 and 10, c‑FLIPL and procaspase-8 itself (1, 5  6). Human procaspase‑8a is a 54‑56 kDa, 479 amino acid (aa) protein (4, 7, 8, 9). It contains two N‑terminal death domains (aa 1‑177), followed by a catalytic site that utilizes His317Gly318 plus Cys360. Normally, it is an inactive, cytosolic monomer (1, 10, 11). But following death‑domain (DD) containing receptor oligomerization, Caspase‑8 is recruited to the death-inducing signaling complex (DISC) that forms around the death domains of the oligomerized receptor (12). FADD/CAP-1 is recruited first, followed by procaspase‑8/CAP‑4 and, possibly, c‑FLIPL and procaspase‑10 (12). The recruitment, or concentration, of procaspase-8 induces homodimerization. This act alone is sufficient for activation. However, the activity level is modest at best, and appears to be directed towards either itself, or c‑FLIPL, which is known to form a functional heterodimer with procaspase‑8 (5, 11). When directed towards itself, autocleavage occurs first between Asp374Ser375, generating a 43 kDa (p43) N‑terminal (aa 1‑374) and an 11 kDa C‑terminal (aa 375‑479) fragment. The C‑terminus is further cleaved between Asp384Leu385 to generate a mature p10 subunit (aa 385‑479). The p43 subunit is next cleaved twice, once between Asp216Ser217, and again between Asp210Ser211 to generate a 26 kDa DD‑containing prodomain (aa 1‑210) with an additional 18 kDa mature p18 subunit (aa 217‑374) (12). p18 and p10 noncovalently associate to form a 28 kDa heterodimer, which subsequently associates with another p18:p10 heterodimer to form an active, mature caspase‑8 molecule. This leaves the DISC to act on downstream apoptotic procaspases. In the event procaspase‑8 comes to the DISC complexed with c-FLIPL, c-FLIPL will be cleaved by procaspase‑8, generating a p43 fragment that is analogous to the Caspase‑8 p43 subunit. This fragment, however, appears not to be an intermediate in a proteolytic cascade. Rather, it serves as a functional subunit, interacting with TRAF2 and activating NF kappa B. This may account for many of the nonapoptotic activities associated with Caspase‑8 (5, 6, 13). Mature human and mouse Caspase‑8a heterodimers are 73% aa identical (14).

全称：Caspase-8 (CASP8)

说明书：待上传