Anti-DKK1 Antibody, Rabbit Polyclonal
产品编号：PA00662HuA10
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规格 50uL 100uL 200uL 可选
产品名称：Anti-DKK1 Antibody, Rabbit Polyclonal
经验证的应用：/
交叉反应：/
特异性：human DKK1
免疫原：Recombinant human DKK1 protein, fragment Thr32~His266; UniprotKB: O94907
制备方法：Produced in rabbits immunized with human DKK1, and purified by antigen affinity chromatography
来源：Polyclonal Rabbit IgG
纯化：Immunogen affinity purified
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物：Unconjugated
状态：Liquid
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片：
Figure1.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas sections labelling DKK1 with purified PA00662HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution (EDTA, pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
别称：SK, Dickkopf Homolog 1(Xenopus Laevis)
背景信息：Dkk-1. Dickkopf related protein 1 (Dkk-1) is the founding member of the Dickkopf family of proteins that includes Dkk-1, -2, -3, -4, and a related protein, Soggy (1, 2). Dkk proteins are secreted proteins that contain two conserved cysteine-rich domains (CRDs) separated by a linker region. Each domain contains ten cysteine residues (1-3). Mature human Dkk-1 is a 40 kDa glycosylated protein that shares 83% amino acid sequence identity with mouse and rat Dkk-1. Dkk-1 is a well-characterized antagonist of Wnt/beta-Catenin signaling (1, 2, 4, 5). This pathway is activated by Wnt engagement of a receptor complex composed of the Frizzled proteins and one of two low-density lipoprotein receptor-related proteins, LRP-5 or LRP-6 (4). Crystallographic studies have shown that Dkk-1 interacts with LRP-6 as a bipartite inhibitor, with both CRDs binding the extracellular domain of LRP-6 simultaneously (4, 6-8). Dkk-1/LRP-6/Krm2 complex internalization has been shown to down-regulate Wnt signaling (4, 9). Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development (10, 11). Other sites of expression include developing neurons, hair follicles, and the retina (12, 13). The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and homeostasis (14). Insufficient or excess Dkk-1 activity in bone results in increased or decreased bone density, respectively (12, 15). In adults, Dkk-1 is expressed in osteoblasts and osteocytes, and neurons. Cerebral ischemia induces Dkk-1 expression, which contributes to neuronal cell death (16). Dkk-1 also likely has a complex role in cancer, as it has been shown to act as a tumor suppressor and also to promote metastasis (17-19)
全称：Dickkopf-related protein 1 (DKK1)
说明书：待上传