Anti-AIF1 Antibody, Rabbit Polyclonal

产品编号：PA00042HuA20
   
$ 询价

规格     50uL  100uL  200uL

产品名称：Anti-AIF1 Antibody, Rabbit Polyclonal

经验证的应用：WB
 
交叉反应：/
 
特异性：human AIF1
 
免疫原：Recombinant human AIF1 protein, fragment Met1~Pro147; UniprotKB: P55008
 
制备方法：Produced in rabbits immunized with human AIF1, and purified by antigen affinity chromatography.

来源：Polyclonal Rabbit IgG
 
纯化：Immunogen affinity purified
 
缓冲液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物：Unconjugated
 
状态：Liquid
 
运输方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片：






Figure1.Western blot analysis of extracts of various cell lines  using AIFI antibody (PA00042HuA20) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 6s.












 


Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum  sections labelling AIF1 with purified PA00042HuA20 at 10ug/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0).  Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.



 



Figure3.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum  sections labelling AIF1 with purified PA00042HuA20 at 10ug/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0).  Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.

 
 
别称：AIF1, IRT1, Daintain, Protein G1, Allograft Inflammatory Factor 1
 
背景信息：AIF1, also named as G1, IBA1 or daintain/AIF-1, is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Despite a lack of detailed knowledge on the in vivo physiological functions of AIF-1, there is growing evidence that shows its aberrant expression contributes to the pathogenesis of many autoimmune diseases, including rheumatoid arthritis. (20944424). AIF1 is an actin-binding protein that enhances membrane ruffling and RAC activation. It enhances lymphocyte migration and the actin-bundling activity of LCP1. AIF1 plays a role in RAC signaling and in phagocytosis and a role in vascular inflammation. AIF1 promotes the proliferation of vascular smooth muscle cells and of T-lymphocytes. It is also as a microglial cell marker.
 
全称：Allograft inflammatory factor 1 (AIF1)
 
说明书：待上传