Anti-CSTB Antibody, Rabbit Polyclonal

产品编号:PA00729HuA10
   
询价

规格     50uL  100uL  200uL
产品名称Anti-CSTB Antibody, Rabbit Polyclonal

经验证的应用WB/ICC
 
交叉反应Hu
 
特异性human CSTB
 
免疫原Recombinant human CSTB protein, fragment Met2~Phe98; UniprotKB: P04080
 
制备方法Produced in rabbits immunized with human CSTB, and purified by antigen affinity chromatography

来源Polyclonal Rabbit IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
状态Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:






Figure1.Western blot analysis of extracts of various cell lines  using CSTB antibody (PA00729HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.









Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon  sections labelling CSTB with purified PA00729HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution  (EDTA, pH 9.0).  Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.


别称Cys-B, CST6, EPM1, PME, STFB, CPI-B, Stefin B, Liver thiol proteinase inhibitor
 
背景信息Cystatin B. Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Mutations in the Cystatin B gene is the cause of progressive myoclonus epilepsy (EPM1) (3). Because of its expression patterns, Cystatin B can be used as a marker for certain cancers, such as glioblastoma tumors (4). It readily forms amyloid fibrils in vitro (5). The human Cystatin B consists of 98 amino acid residues (3)
 
全称Cystatin-B (CSTB)
 
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