Anti-CSTB Antibody, Rabbit Polyclonal
产品编号:PA00729HuA10
$ 询价
规格 50uL 100uL 200uL
产品名称:Anti-CSTB Antibody, Rabbit Polyclonal
经验证的应用:WB/ICC
交叉反应:Hu
特异性:human CSTB
免疫原:Recombinant human CSTB protein, fragment Met2~Phe98; UniprotKB: P04080
制备方法:Produced in rabbits immunized with human CSTB, and purified by antigen affinity chromatography
来源:Polyclonal Rabbit IgG
纯化:Immunogen affinity purified
缓冲液:Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物:Unconjugated
状态:Liquid
运输方式:This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片:
Figure1.Western blot analysis of extracts of various cell lines using CSTB antibody (PA00729HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon sections labelling CSTB with purified PA00729HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution (EDTA, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
别称:Cys-B, CST6, EPM1, PME, STFB, CPI-B, Stefin B, Liver thiol proteinase inhibitor
背景信息:Cystatin B. Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Mutations in the Cystatin B gene is the cause of progressive myoclonus epilepsy (EPM1) (3). Because of its expression patterns, Cystatin B can be used as a marker for certain cancers, such as glioblastoma tumors (4). It readily forms amyloid fibrils in vitro (5). The human Cystatin B consists of 98 amino acid residues (3)
全称:Cystatin-B (CSTB)
说明书:待上传
经验证的应用:WB/ICC
交叉反应:Hu
特异性:human CSTB
免疫原:Recombinant human CSTB protein, fragment Met2~Phe98; UniprotKB: P04080
制备方法:Produced in rabbits immunized with human CSTB, and purified by antigen affinity chromatography
来源:Polyclonal Rabbit IgG
纯化:Immunogen affinity purified
缓冲液:Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物:Unconjugated
状态:Liquid
运输方式:This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片:
Figure1.Western blot analysis of extracts of various cell lines using CSTB antibody (PA00729HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60s.
Figure2.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon sections labelling CSTB with purified PA00729HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution (EDTA, pH 9.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
别称:Cys-B, CST6, EPM1, PME, STFB, CPI-B, Stefin B, Liver thiol proteinase inhibitor
背景信息:Cystatin B. Cystatin B, also called stefin B or liver thiol proteinase inhibitor, is a member of family 1 of the cystatin superfamily (1). Like Cystatin A, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (2). Mutations in the Cystatin B gene is the cause of progressive myoclonus epilepsy (EPM1) (3). Because of its expression patterns, Cystatin B can be used as a marker for certain cancers, such as glioblastoma tumors (4). It readily forms amyloid fibrils in vitro (5). The human Cystatin B consists of 98 amino acid residues (3)
全称:Cystatin-B (CSTB)
说明书:待上传