Anti-ALOX12 Antibody, Rabbit Polyclonal

产品编号:PA00052HuA20
   
询价

规格     50uL  100uL  200uL


说明书
产品名称Anti-ALOX12 Antibody, Rabbit Polyclonal

经验证的应用WB/IHC
 
交叉反应Human
 
特异性human ALOX12
 
免疫原Recombinant human ALOX12 protein, fragment Gly2~Thr405; UniprotKB: P18054
 
制备方法Produced in rabbits immunized with human ALOX12, and purified by antigen affinity chromatography.

来源Polyclonal Rabbit IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
状态Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:






Figure1.Application in WB
Western blot analysis of extracts of various cell lines and tissues, using ALOX12 antibody (PA00052HuA20) at 2μg/mL dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 8s.




 

Figure2.Application in IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart  sections labelling ALOX12 with purified PA00052HuA20 at 10μg/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.

Figure3.Application in IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver  sections labelling ALOX12 with purified PA00052HuA20 at 10μg/ml. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.




别称LOX12, 12-LOX, ALOX-12, 12-LO, 12LPG, LOG12, Lipoxygenase 12, 12S-lipoxygenase, Lipoxin synthase 12-LO, Platelet-type lipoxygenase 12
 
背景信息/
 
全称Polyunsaturated fatty acid lipoxygenase ALOX12 (ALOX12)