Anti-ME2 Antibody, Rabbit Polyclonal

产品编号:PA00844HuA10
   
询价

规格     50uL  100uL  200uL

产品名称Anti-ME2 Antibody, Rabbit Polyclonal

经验证的应用WB
 
交叉反应/
 
特异性human ME2
 
免疫原Recombinant human ME2 protein, fragment Gly220~Ala426; UniprotKB: P23368
 
制备方法Produced in rabbits immunized with human ME2, and purified by antigen affinity chromatography.

来源Polyclonal Rabbit IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
状态Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:



Figure1.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human  colon sections labelling ME2 with purified PA00844HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using EDTA buffer (pH 9.0).  Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.







别称ODS1
 
背景信息ME2(malic enzyme 2) is also named as NAD-ME and belongs to the malic enzymes family. ME1, ME2 are important for NADPH production, lipogenesis and glutamine metabolism, but ME2 has a more profound effect. ME2 is a genome-coded mitochondrial enzyme that converts malate to pyruvate and is involved in neuronal synthesis of the neurotransmitter 纬-aminobutyric acid (GABA)(PMID:15532013). This protein has 2 isoforms produced by alternative splicing with the MW of 65 kDa and 54 kDa.
 
全称NAD-dependent malic enzyme, mitochondrial (ME2)
 
说明书
说明书