Anti-WT1 Antibody, Rabbit Polyclonal
产品编号:PA00357HuA10
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规格 50uL 100uL 200uL
产品名称:Anti-WT1 Antibody, Rabbit Polyclonal
经验证的应用:ICC/WB
交叉反应:/
特异性:human WT1
免疫原:Recombinant human WT1 protein, fragment Leu310~Leu449; UniprotKB: P19544
制备方法:Produced in rabbits immunized with human WT1, and purified by antigen affinity chromatography.
来源:Polyclonal Rabbit IgG
纯化:Immunogen affinity purified
缓冲液:Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶联物:Unconjugated
状态:Liquid
运输方式:This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
储存条件:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
图片:
Figure1.Western blot analysis of extracts of various cell lines using WT1 antibody (PA00357HuA10) at 1:1000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 10% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 7s.
别称:WAGR, GUD, WIT-2, WT33
背景信息:The WT1 gene encodes a zinc finger DNA-binding protein that acts as a transcriptional activator or repressor depending on the cellular or chromosomal context, and it is required for normal formation of the genitourinary system and mesothelial tissues. WT1 inhibits apoptosis through p53 and Bcl-2 and also inhibits the differentiation of leukemic cells. Function of WT1 may be isoform-specific: isoforms lacking the KTS motif may act as transcription factors. Isoforms containing the KTS motif may bind mRNA and play a role in mRNA metabolism or splicing. Isoform 1 has lower affinity for DNA, and can bind RNA. WT1 exists some isoforms with the range of molecular weight is 33-50 kDa.
全称:Wilms tumor protein (WT1)
说明书:待上传