Anti-PARP1 Antibody, Rabbit Polyclonal

产品编号:PA00407HuA10
   
询价

规格     50uL  100uL  200uL
产品名称Anti-PARP1 Antibody, Rabbit Polyclonal

经验证的应用WB/IHC/ICC
 
交叉反应Hu
 
特异性human PARP1
 
免疫原Recombinant human PARP1 protein, fragment Asp788~Trp1014; UniprotKB: P09874

制备方法Produced in rabbits immunized with human PARP1, and purified by antigen affinity chromatography.

来源Polyclonal Rabbit IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
状态Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:






Figure1.Western blot analysis of extracts of various cell lines and cell lines HeLa (Human epithelial cells from cervix adenocarcinoma) treated with 1uM staurosporine for 4 hours,and HCT 116 (human colorectal carcinoma cell line) treated with 30 µM cisplatin for 24 hourstreated , using PARP antibody (PA00407HuA10) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)  at 1:10000 dilution.





















别称PARP1, ADPRT, ADPRT1, PPOL, pADPRT-1, ADP-ribosyltransferase diphtheria toxin-like 1, NAD(+) ADP-ribosyltransferase 1
 
背景信息PARP1 (poly(ADP-ribose) polymerase 1) is a nuclear enzyme catalyzing the poly(ADP-ribosyl)ation of many key proteins in vivo. The normal function of PARP1 is the routine repair of DNA damage. Activated by DNA strand breaks, the PARP1 is cleaved into an 85 to 89-kDa COOH-terminal fragment and a 24-kDa NH2-terminal peptide by caspases during the apoptotic process. The appearance of PARP fragments is commonly considered as an important biomarker of apoptosis. In addition to caspases, other proteases like calpains, cathepsins, granzymes and matrix metalloproteinases (MMPs) have also been reported to cleave PARP1 and gave rise to fragments ranging from 42-89-kDa. This antibody was generated against the C-terminal region of human PARP1 and it recognizes the full-length as well as the cleavage of the PARP1.

全称Poly [ADP-ribose] polymerase 1 (PARP1)

说明书
说明书