Anti-ACTN1 Antibody, Rabbit Polyclonal

产品编号:PA00060HuA20
   
询价

规格     50uL  100uL  200uL

说明书
产品名称Anti-ACTN1 Antibody, Rabbit Polyclonal

经验证的应用WB/IHC
 
交叉反应Human
 
特异性endogenous levels of total ACTN1 protein
 
免疫原Recombinant human ACTN1 protein, fragment Asn272~Thr500; UniprotKB: P12814
 
制备方法Produced in rabbits immunized with human ACTN1, and purified by antigen affinity chromatography.

来源Polyclonal Rabbit IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
状态Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:






Figure1.Application in WB
Western blot analysis of extracts of various cell lines and tissues, using ACTN1 antibody (PA00060HuA20) at 1μg/mL. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20 s.






 


Figure2.Application in IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney sections labelling ACTN1 with purified PA00060HuA20 at 10μg/mL. Heat mediated antigen retrieval was performed using citrate buffer (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control.
 



 
别称Alpha-actinin cytoskeletal isoform, F-actin cross-linking protein, Non-muscle alpha-actinin-1
 
背景信息Alpha Actinins are highly conserved actin-binding proteins that form antiparallel homodimers with the actin-binding domains on each end of the molecule, allowing crosslinking of actin molecules. There are four alpha actinin isoforms identified: two ubiquitously expressed cytoskeletal isoforms (ACTN1 and ACTN4) and two striated muscle-specific sarcomeric isoforms (ACTN2 and ACTN3). ACTN1 is also alternatively spliced to form smooth muscle or brain specific isoforms. This antibody recognize endogenous ACTN1, while it may cross-react with ACTN2 and ACTN4.
 
全称Alpha-actinin-1 (ACTN1)