Anti-CD68 Antibody, Rabbit Polyclonal

产品编号:PA00065HuA10
   
询价

规格     50uL  100uL  200uL

说明书
 

产品名称Anti-CD68 Antibody, Rabbit Polyclonal

经验证的应用WB/ICC
 
交叉反应/
 
特异性human CD68
 
免疫原A synthetic peptide of  human CD68; UniprotKB: P34810
 
制备方法Produced in rabbits immunized with human CD68, and purified by antigen affinity chromatography.

来源Polyclonal Rabbit IgG
 
纯化Immunogen affinity purified
 
缓冲液Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
 
偶联物Unconjugated
 
状态Liquid
 
运输方式This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
 
储存条件This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

图片:


Figure1.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma sections labelling CD68 with purified PA00065HuA10 at 10ug/ml. Heat mediated antigen retrieval was performed using EDTA buffer (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1/4000 dilution. PBS instead of the primary antibody was used as the negative control. 











别称CD68, GP110, Macrosialin, Macrophage Antigen CD68
 
背景信息CD68/SR-D1. CD68, also called Scavenger Receptor D1(SR-D1) and LAMP-4, is a heavily glycosylated type I transmembrane glycoproteinthat belongs to the LAMP family (1). Human CD68 contains 354 amino acids (aa), witha 21 aa signal sequence and a 298 aa extracellular domain (ECD), a 25 aatransmembrane domain, and a 10 aa cytoplasmic domain. The ECD of CD68 has 9 glycosylation sites and a mucin-like region in aa 23-140. Within the ECD, humanCD68 shares a 65% and 67% amino acid sequence identity with that of mouse andrat CD68, respectively. CD68 is expressed onmonocytes and tissue macrophages and serves as a scavenger receptor foroxidized LDL (2).  In contrast to other members of the LAMP-family ofglycoproteins which are ubiquitously expressed in all cell types, CD68 appearedto have a more restricted, cell-type specific pattern of expression (3 ,4). CD68 is alsoa biomarker of rheumatoid arthritis and Hodgkin's lymphoma (5, 6). CD68influences the functions of cells through NF-kB/focal adhesion kinase pathway (7).In addition, CD68 on macrophages binds tightly to both S100A8 and S100A9 toenhance the cell immunity (8).
 
全称Macrosialin (CD68)